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pbluescript ii sk (+) plasmid dna  (Agilent technologies)


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    Structured Review

    Agilent technologies pbluescript ii sk (+) plasmid dna
    Pbluescript Ii Sk (+) Plasmid Dna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii+sk+(+)+plasmid+dna/pm36462794-96-1-7
    Average 90 stars, based on 1 article reviews
    pbluescript ii sk (+) plasmid dna - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Plasmid Preparation:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Derivative Assay:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Sequencing:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Recombinant:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Software:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Purification:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    BAC Assay:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Modification:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    shRNA:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    DNA Labeling:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Staining:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Imaging:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Light Microscopy:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Knock-In:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Incubation:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Migration:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Electrophoresis:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Binding Assay:

    Article Title: Somatic cell reprogramming
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, Calif.).

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: Generation of Aldh1a1-P2A-CreERT2 knock-in mouse line An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Magnetic Tweezers Data Part 1/2, related to Figures 1, 2, 3 and 4 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/7wdr63x5h9.1 Magnetic Tweezers Data Part 2/2, related to Figures 5, 6, S4, S5 and S7 This paper; Mendeley Data Mendeley Data: https://doi.org/10.17632/4pdz8cv9cz.1 Python code for data analysis This paper; Moreno-Herrero Lab Github Repository https://github.com/Moreno-HerreroLab/ MTDataProcessing/ Experimental models: Cell lines Sf9 cells: Spodoptera frugiperda cell line derived from strain IPLB-Sf-21-AE Thermo Fisher Scientific Cat#11496015 High Five cells: cell line BTI-TN-5B1-4, clonal isolate from Trichoplusia ni cell line Thermo Fisher Scientific Cat#B85502 Oligonucleotides DNA oligonucleotides used in this work, see Table S1 This paper N/A MT central dsDNA fragment (4292 bp), see Table S2 This paper N/A AFM dsDNA fragment (441 bp), see Table S2 This paper N/A NIHCOLE lncRNA, see Table S3 for sequence Unfried et al.38 N/A Antisense NIHCOLE RNA, see Table S3 for sequence This paper N/A SM2 and SM3 RNAs, see Table S3 for sequences Unfried et al. 202138 N/A Recombinant DNA Plasmid: pBluescript II SK+ Stratagene Cat#212205 Plasmid; 71dir v3.pBluescript SK + -2 gaps This paper N/A Plasmid: pNLrep Luzzietti et al.54 N/A Plasmid: pSP73-JY0 Fili et al.55 N/A Plasmid: pcDNA3.1-NIHCOLE Unfried et al.38 N/A Plasmid: pcDNA3.1-antisense NIHCOLE This paper N/A Software and algorithms SnapGene – Molecular biology software SnapGene software www.snapgene.com Python version 3.9 Python Software Foundation www.python.org WSxM – AFM processing software Horcas et al.56 www.wsxm.eu Other DNA sequence analysis Unidad de Genómica CAI Genómica y Proteómica Facultad CC Biológicas – UCM https://cai.ucm.es/en/ biological-techniques/ genomics/ Bio-Rad Gel DocTM 2000 Bio-Rad N/A LabChip GX Touch 24 Nucleic Acid Analyzer PerkinElmer Cat#CLS138162 Article ll OPEN ACCESS

    Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning
    Article Snippet: An ~8.0 kb genomic fragment containing the Aldh1a1 locus was digested from BAC clone B6Ng01-066I24 (RIKEN BRC) by KpnI and AvrII , and subcloned into modified pBlueScript II SK (+) vector (Agilent Technologies) for further modifications.

    Article Title: Blue light exposure enhances oxidative stress, causes DNA damage, and induces apoptosis signaling in B16F1 melanoma cells.
    Article Snippet: Studies have shown that visible light, specifically blue light, adversely affects cells, tissues, organs, and organisms.. We investigated the effect of blue light on apoptosis, DNA integrity, and transcription of apoptotic and melanogenic genes using B16F1 melanoma cells.. In this study, cells were irradiated with 2–50 W/m2 blue light (465 nm) for several time duration.

    Article Title: Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2
    Article Snippet: All genomic fragments and the cassette were cloned into the multiple cloning site (MCS) of a cloning vector, pBluescript® SK II (GenBank Accession Number X52328; Stratagene; La Jolla, CA).

    Article Title: Modulation of cyclobutane thymine photodimer formation in T 11 -tracts in rotationally phased nucleosome core particles and DNA minicircles
    Article Snippet: The dimeric DNA was then cloned into pBlueScript II SK- vector DNA (Agilent) that had been restricted with EcoRI and then dephosphorylated with calf intestinal phosphatase (CIP) (New England Biolabs) in 100 mM NaCl, 50 mM Tris–HCl (pH 7.5), 10 mM MgCl 2 and 1 mM DTT, for 30 min, at 37°C, to prevent re-circularization.

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice
    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).



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    PMB had high affinity for DNA and inhibited electrophoretic migration of plasmid at higher concentrations. (A) 60 ng of pBlueScript II DNA incubated with PMB (12.5–500 μM) in a topoisomerase I buffer for 2 h at 37 °C. Samples were resolved by electrophoresis. (B) Chemical structure of PMB with potential DNA binding sites (including the Dab residues) highlighted.

    Journal: Archives of toxicology

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice

    doi: 10.1007/s00204-018-2192-1

    Figure Lengend Snippet: PMB had high affinity for DNA and inhibited electrophoretic migration of plasmid at higher concentrations. (A) 60 ng of pBlueScript II DNA incubated with PMB (12.5–500 μM) in a topoisomerase I buffer for 2 h at 37 °C. Samples were resolved by electrophoresis. (B) Chemical structure of PMB with potential DNA binding sites (including the Dab residues) highlighted.

    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Techniques: Migration, Plasmid Preparation, Incubation, Electrophoresis, Binding Assay